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KMID : 0603820100160040331
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Journal of Experimental & Biomedical Science
2010 Volume.16 No. 4 p.331 ~ p.339
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Photodynamic Therapy for Methicillin-resistant Staphylococcus aureus with High-level Mupirocin Resistance using 630 ㎚ Light-emitting Diode
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Kwon Pil-Seung
Kim Jin-Kyung
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Abstract
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This study aims to evaluate the effect of Photodynamic Therapy (PDT) against methicillin-resistant Staphylococcus aureus with high-level mupirocin resistance (Hi-Mup MRSA). To examine the antimicrobial effect of photogemmediated PDT against Hi-Mup MRSA, CFU quantifications, bacteria cell viability tests, and disk diffusion antimicrobial susceptibility tests were evaluated. In addition, one of PDT mechanisms was investigated by accumulating photogem (10 ㎍/㎖) in Hi-Mup MRSA. Photogem-mediated PDT properly inhibited the colony formation of Hi-Mup MRSA. Viable bacteria decreased greatly after a PDT application with photogem 10 ㎍/㎖ at energy density 15 J/㎠. The diameter of the inhibition zone around susceptible disks increased after PDT. In addition, we confirmed the accumulation of photogem in bacteria through fluorescent images. These results demonstrated that excellent photosensitization of Hi-Mup MRSA can be achieved using photogem with 630 nm LED irradiation. Thus, PDT may make survival Hi-Mup MRSA inactive.
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KEYWORD
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Mupirocin, Methicillin-resistant Staphylococcus aureus, Photodynamic therapy
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